Non-destructive methodology in comparative physiology of buckwheat genotypes within the different origin

Authors

  • Oksana Sytar Department of Plant Physiology, Slovak University of Agriculture, Nitra, A. Hlinku 2, 94976 Nitra, Slovak Republic; Plant Biology Department, Taras Shevchenko National University of Kyiv, Institute of Biology, Volodymyrska str., 64, Kyiv01033, Ukraine
  • Klaudia Bruckova Department of Plant Physiology, Slovak University of Agriculture, Nitra, A. Hlinku 2, 94976 Nitra, Slovak Republic
  • Alyona Plotnitskaya Laboratory of Quality and Safety of Products of Agro Industrial Complex. Mashynobudivnykiv Street Building 7, Chabanyvillage, Kyiv-Svyatoshinsky district, Kyiv region, 08162, Ukraine
  • Marek Zivcak Department of Plant Physiology, Slovak University of Agriculture, Nitra, A. Hlinku 2, 94976 Nitra, Slovak Republic
  • Marian Brestic Department of Plant Physiology, Slovak University of Agriculture, Nitra, A. Hlinku 2, 94976 Nitra, Slovak Republic

DOI:

https://doi.org/10.3986/fag0007

Abstract

In the presented study has been used non-destructive method for prescreening of flavonoids, anthocyanins and pig­ments from early stage of growth till flowering period of buckwheat genotypes of different origin. The similar increasingtendency in the changes of FLAV, ANTH and MFI indexes of Chinese genotypes compared to the tendency of Ukrainiangenotypes has been observed. Genotypes of F.tataricumcompared to the genotypes of F.esculentumhave been shownto lower ANTH index during seedling growth.SFR indexwhich relates to chlorophyll concentration shown differentdynamic between Ukrainian and Chinese genotypes. In the middle phase 30 days after sowing (DAS) of growth hasbeen discovered significant increase of SFR index almost in all experimental genotypes. On 36 DAS just two Ukrainiangenotypes which characterized also high ANTH and MFI indexes has been kept tendency to increase SFR index. HPLCanalysis of experimental samples found that presence of p-anisic acid was typical for F.tataricumspecies compared tothe experimental cultivars of F.esculentumof both origin. Buckwheat genotypes of different origin can vary in flavonoid,anthocyanins and pigments content during stages of growth, but changes in their contents can be similar for represent­atives of the same origin.

Received: March 14, 2019; accepted: May 25, 2019

Keywords: chlorophyll fluorescence, phenolic acid, non-destructive measurement, flavonoids, anthocyanins

Author Biography

Oksana Sytar, Department of Plant Physiology, Slovak University of Agriculture, Nitra, A. Hlinku 2, 94976 Nitra, Slovak Republic; Plant Biology Department, Taras Shevchenko National University of Kyiv, Institute of Biology, Volodymyrska str., 64, Kyiv01033, Ukraine

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2                                Plant Biology Department, Taras Shevchenko National University of Kyiv, Institute of Biology, Volodymyrska str., 64, Kyiv01033, Ukraine

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E-mail address of corresponding author:

Oksana SYTAR: Coauthors:

Marek ZIVCAK, email: , Alyona PLOTNITSKAYA, email: ,

Klaudia BRUCKOVA, email: , Marian BRESTIC, email: 

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Issue

Vol. 36 No. 1 (2019)

Section

Articles